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Practical Plans

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Practical plans are displayed at least one week in advance so as to enable students to get themselves familiarized with next week's experiments, complete all background reading and get maximum from their practical experience. Students are required to complete any assignments and comply with instructions provided here. A copy of this material is also displayed on departmental notice board.

The General schedule of uploading plan for each class is as follows. In case for some reason the plan is not uploaded on scheduled day, students must check departmental notice boards and contact their practical in-charge teachers as indicated below.

Class Teacher In-charge Weekly upload Day
F Y B Sc

Mr. V Tupe

 Wednesday
S Y B Sc Mrs. U Palan Monday
T Y B Sc Mrs. L Phadke Friday
T Y B Sc A/C Mrs. A Lokur Monday

Class wise Detailed Plans

F Y B Sc Practical Plan:

15th and 16thJan 2007

 

Repeat from last Week

  • Enumeration of organisms using Haemocytometer (Yeast isolate)
  • Biofertilizers – Isolation of Phosphate solubilizers – checking colony characteristics
  • Discussion of results of growth curve

 

Plan for this week

  • Enumeration of organisms using the viable count methods-
    • Determine the microbial load of the given sample using pour plate method & comparison with spread plate method.
  • Preservation of bacteria:

o        After preservation for appropriate period confirm the purity of the culture visibility

·         Isolation of Actnomycetes.

·         Study of Molds: - Study the morphology of molds by slide culture technique

  • Studying morphological characteristics of isolated microorganisms

o        Looking for bacterial flagella by Kirk Patrick’s method

·         Breed’s count technique.

Biofertilizer preparation – preparation of culture stocks (symbiotic / non-symbiotic / phosphate solubilizer) for mixing into biofertilizer, formulation of biofertilizer and testing it’s efficacy on cucumber seedlings.

S Y B Sc Practical Plan: 11th Jan to 13th Jan 2007

1. Strip plate technique

2. Lecithinase

3. Phagocytosis

4. COD

5. Enzymology Calculations

6. BOD - 5 day reading

T Y B Sc Practical Plan: 8th to 11th Jan 2007

1. Chemical Assay of Penicillin
2. Study of Saurkraut (contd.)

T Y Applied Component (Biotechnology) Practical Plan : 12th Jan 2007

1. Assay of beta galactosidase (repeat)

2. Assay of immobilized invertase

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Last modified: December 18, 2006
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